Compared with normal individuals, the level of PITX1 gene expression was significantly higher in invasive breast carcinoma, invasive ductal breast carcinoma, invasive lobular breast carcinoma, ductal breast carcinoma, medullary breast carcinoma, invasive ductal and invasive lobular breast carcinoma and tubular breast carcinoma (Figure 2A–K, P=9.96E-28, 3.35E-42, 2.88E-13, 1.18E-7, 2.70E-9, 2.49E-1.4, 1.85E-30, 1.60E-39, 1.48E-76, 1.41E-5 and 3.43E-1.4). This evidence concerns the gene PITX1 and breast ductal adenocarcinoma.