The present results revealed that higher expression of PITX1 was located in different histological classification of breast cancer with respect to normal individuals, including invasive breast carcinoma, invasive ductal breast carcinoma, invasive lobular breast carcinoma, ductal breast carcinoma, medullary breast carcinoma, invasive ductal and invasive lobular breast carcinoma and tubular breast carcinoma. The gene discussed is PITX1; the disease is breast cancer.