In the present study, Mang‐NPs inhibited the expression of stem cells markers (CD133, CD44, Musashi and LGR5) and pluripotency maintaining factors (Oct‐4, Sox‐2, KLF‐4, c‐Myc and Nanog) in CSCs, suggesting that Mang‐NPs can inhibit CSC characteristics and stem cell population in CRC. The gene discussed is NANOG; the disease is colorectal carcinoma.