In male C57BL/6 J mice fed a 10 week long HFD to induce obesity, restricting methionine increases plasmatic but also skeletal muscle adiponectin, downregulates the mTOR pathway and upregulates MAPK and IRS-1 mRNA levels in gastrocnemius muscle, leading to an improvement in insulin sensitivity [92]. This evidence concerns the gene INS and obesity due to melanocortin 4 receptor deficiency.