HIF1A and familial pancreatic carcinoma: To assess whether this HIF-1α protein localization in normoxic and mild hypoxic areas is also observed in pancreatic cancer xenografts, we performed immunostaining of HIF-1α protein and pimonidazole, which forms adducts of thiol-containing proteins in cells with <10 mm Hg of pO2 [19] in tumors of orthotopically injected human pancreatic cancer AsPC-1 cells that were also perfused with Hoechst 33342 via intravenous injection to detect cells near functional blood vessels.