PEG10 and hepatocellular carcinoma: Furthermore, western blot analysis showed that the upregulation of miR-27a-3p and miR-34a-5p in HepG2 and MHCC-97H cells triggered a significant decrease in PEG10 protein expression, while downregulation of miR-27a-3p and miR-34a-5p increased PEG10 expression in HCC cells (Fig. 5c).