In our study, ACE2 of Chinese pangolin, Sunda pangolin, and white-bellied pangolin had low or very low binding score for SARS-CoV-2 S. Binding of pangolin ACE2 to SARS-CoV-2 S was predicted using molecular binding simulations (67); however, neither experimental infection nor in vitro infection with SARS-CoV-2 has been reported for pangolins. This evidence concerns the gene ACE2 and infection.