Moreover, our biosensor has several advantages, including high binding affinity of antigen-antibody interaction, small size, light-independent point-of-care diagnosis, preventing the denaturation of target proteins, and good selectivity for aβ 1-42 in HS containing CRP, TNF-α, and insulin, which are the benefits of evaluation criteria for the practical application and the early diagnosis of AD. Here, TNF is linked to Alzheimer disease.