We evaluated the mRNA expression levels of ESR1 (ER-α) and ESR2 (ER-β) genes in astrocytoma samples with different histological grades from the data obtained of the low-grade gliomas (LGG) and glioblastoma (GBM) projects from the TCGA repository, as well as samples of healthy cerebral cortex in the GTEx database. Here, ESR1 is linked to glioblastoma.