We evaluated the mRNA expression levels of ESR1 (ER-α) and ESR2 (ER-β) genes in astrocytoma samples with different histological grades from the data obtained of the low-grade gliomas (LGG) and glioblastoma (GBM) projects from the TCGA repository, as well as samples of healthy cerebral cortex in the GTEx database. Here, ESR2 is linked to central nervous system cancer.