To evaluate induction of apoptosis in the colon cancer cells upon MS13 treatment, morphological changes related to cell death was first observed by examining the Annexin V-FITC/PI-stained colon cancer cells under the fluorescence microscope at EC50 and 2XEC50 of MS13 concentration for 24, 48 and 72 h. This evidence concerns the gene ANXA5 and malignant colon neoplasm.