Therefore the aim of the present study was to determine the cytotoxicity, anti-proliferative and apoptotic activity of 1,5-bis(4-hydroxy-3- methoxyphenyl)-1, 4-pentadiene-3-one which is MS13 on human primary (SW480) and metastatic (SW620) colon cancer cells carrying KRAS and p53 mutations and also undergone CIN. This evidence concerns the gene KRAS and malignant colon neoplasm.