Therefore, the cause of cell death in correlation to the reduced number of viable colon cancer cells upon MS13 treatment was investigated via apoptosis by quantification of apoptotic cells, measuring caspase-3 activity and Bcl-2 protein level at dosages of EC50 and 2XEC50 on SW480 and SW620 cells for 24, 48, and 72 h. The gene discussed is CASP3; the disease is malignant colon neoplasm.