To this end, MDA-MB-231 breast cancer cells stably transfected with either the control (shLuc) or CAV1-specific (shCAV1) short hairpin RNA-expressing constructs were additionally transfected with the reporter plasmid pGL3-HRE and exposed to normoxia or hypoxia in order to evaluate changes in HIF transcriptional activity. Here, CAV1 is linked to breast cancer.