Based on these observations, we chose to work with HT29(US) cells, a human colon cancer cell line with enhanced metastatic potential compared with commercially available HT29(ATCC) cells [7], highly metastatic B16F10 murine melanoma cells [9,49] (both with low CAV1 expression) and the human breast cancer cell line MDA-MB-231, which expresses high endogenous levels of CAV1 [50]. The gene discussed is CAV1; the disease is malignant colon neoplasm.