However, when PSA was introduced in the miRNA-based model, it resulted in higher predictive accuracy than PSA as biomarker as previously described [8], both to discriminate PCa from nonmalignant individuals (miR-142-3p + miR-142-5p + PSA model: AUC > 0.85, p < 0.009) and to discriminate benign from malignant tumours (miR-142-3p + miR-142-5p + miR-223-3p + PSA model: AUC > 0.933, p < 0.009) for the three sEV extraction methods (Table 4). This evidence concerns the gene KLK3 and posterior cortical atrophy.