To analyze the serum SCAND3 and Myo1g methylation levels in different groups, the methylation status of HCC patients, HBV (hepatitis B virus)-related liver cirrhosis (LC) patients, benign liver disease (BLD) cases, and healthy controls (HC) were assessed by a methylation-specific polymerase chain reaction (MSP). This evidence concerns the gene SCAND3 and laryngotracheoesophageal cleft.