All of these compounds were tested for their in vitro cytotoxic activity against human cancer cell lines—namely CCRF-CEM, CEM-DNR, HCT116, HCT116 p53−/−, K562, K562-TAX, A549, U2OS, and two noncancerous fibroblasts such as BJ and MRC-5. This evidence concerns the gene TP53 and cancer.