Using control-siRNA vs. ZKSCAN3-siRNA lines, we assessed the functional role of ZKSCAN3 in the proliferation via methyl-thiazolyl-disphenyl-tetrazolium bromide (MTT) assay (Figure 5A), apoptosis via flow cytometry (Figure 5B) and TUNEL assay (Figure 5C), migration via wound-healing assay (Figure 5D), and invasion via transwell invasion assay (Figure 5E) and quantitative PCR for MMP2 (Figure 5F) and MMP9 (Figure 5G) of prostate cancer cells. The gene discussed is MMP2; the disease is prostate cancer.