MMP9 and Familial prostate cancer: Using control-siRNA vs. ZKSCAN3-siRNA lines, we assessed the functional role of ZKSCAN3 in the proliferation via methyl-thiazolyl-disphenyl-tetrazolium bromide (MTT) assay (Figure 5A), apoptosis via flow cytometry (Figure 5B) and TUNEL assay (Figure 5C), migration via wound-healing assay (Figure 5D), and invasion via transwell invasion assay (Figure 5E) and quantitative PCR for MMP2 (Figure 5F) and MMP9 (Figure 5G) of prostate cancer cells.