Next, we assessed the clinical relevance of both new candidates by measuring the gene expression levels of SP8 and FGF8 in an enlarged cohort of 35 HB samples and 11 normal liver specimens via Q-PCR analysis and then associating expression with clinicopathological features such as gender, age at diagnosis, histology, metastasis, vascular invasion, multifocality, PRETEXT stage, and outcome. This evidence concerns the gene FGF8 and hemoglobin measurement.