In this study, two major cytolytic proteins of lytic granules eg. perforin and granzymes and antimicrobial molecules, e.g., granulysin, as well as IFN-γ were upregulated after in vitro PPD or H37Ra stimulation in active TB compared to HIV/TB coinfection before anti-TB treatment which increased after treatment, indicating the importance of these effector molecules in host immune mechanism against tuberculosis. The gene discussed is IFNG; the disease is tuberculosis.