However, after PPD stimulation, there was no change in perforin levels in TB (before treatment, 533 pg/mL; after treatment, 538 pg/mL, p = 0.780, Figure 2c), but decrease in HIV/TB coinfection (before treatment, 631 pg/mL; after treatment, 413 pg/mL, p = 1.000, Figure 2d). This evidence concerns the gene PRF1 and tuberculosis.