According to ELISA data, in subjects with IHD, blood levels were lower for Flt3 ligand (1.56-fold), GM-CSF (2.0-fold), sCD40L (1.7-fold), IL-7 (1.5-fold), MCP-1 (1.76-fold), MIP-1β (1.2-fold), and TNFα (1.26-fold) as compared with subjects without IHD.Thus, significant associations were shown between blood serum cytokine/chemokine measurements by multiplex analysis and ELISA. The gene discussed is CCL4; the disease is myocardial ischemia.