Specifically, we sought to characterize the expression of E2F8 in ovarian cancer tissues and cell lines, to describe its relationship with the cancer prognosis, and to determine the effects of E2F8 knockdown on the proliferation, invasion, and migration of ovarian cancer cells as well as on tumor growth and on the EMT and Notch signaling pathways associated with the cancer. Here, E2F8 is linked to ovarian cancer.