In the present study, the higher insulin and HOMA-IR values observed in the subjects with TSAT<20% are in agreement with some results of the EPIC-InterAct prospective study (Podmore et al., 2016), the KORA F4 study (Huth et al., 2015), and the US NHANES (Cheung et al., 2013), all of them showing associations between low TSAT and prediabetes. The gene discussed is INS; the disease is prediabetes syndrome.