We found that ribosomal proteins, such as ribosomal protein S20 (RPS20), S14 (RPS14), L15 (RPL15), and S14 precursors, which are generally expressed in bradyzoites [29], were highly expressed in the ME49 strain 72 h post-infection, indicating bradyzoite formation in the brain organoids. Here, RPS20 is linked to infection.