To study the effect of pharmacological manipulation of AR signaling in prostate cancer cells, we performed expression profiling of LNCaP cells that were cultured for 72 h in charcoal-stripped fetal bovine serum and stimulated with 10-nM AR agonist, dihydrotestosterone (DHT), or 10 μM of the AR-antagonist casodex for 24 h. Here, AR is linked to prostate cancer.