To sum up, we found that miR-450a-5p can cooperate with gefitinib to inhibit the glioma tumorigenesis through inducing autophagy by regulating EGFR-induced PI3K/AKT/mTOR signaling pathway in glioma cells, thereby enhancing the drug sensitivity of glioma cells to gefitinib. Here, EGFR is linked to central nervous system cancer.