In agreement, Ag-expT-bet+ Th1 cells exhibited higher and sustained mTOR activity, as indicated by p4EBP1 expression [the expression of which, above baseline levels observed in naive T cells, was Torin sensitive (Supplemental Fig. 1E)] and an elevated metabolic signature (c-Myc and pS6) compared with Ag-expT-bet− non-Th1 cells on both days 5 and 15 of infection (Fig. 4E, 4F, Supplemental Fig. 2A). Here, MTOR is linked to infection.