To assess whether in vitro D-RNA expression patterns are recapitulated in vivo, alpha interferon receptor-null (IFN-αR−/−) (A129) mice were infected with 103 PFU of CHIKV AF15561, the parental strain to CHIKV 181/clone 25 (46, 61), and monitored for 4 days post-infection (dpi) until clinical signs such as ruffled fur and hunched posture appeared; serum, skeletal muscle from injected and contralateral limbs, heart, kidney, liver, and spleen were collected upon humane euthanization at 4 dpi. This evidence concerns the gene IFNAR1 and infection.