In this study, we demonstrated for the first time the strong neuroprotective capacity of DIM in an in vivo model of birth asphyxia, as evidenced by the restoration of the weight of the ipsilateral brain hemisphere, the normalization of the number of neurons in rat brains exposed to hypoxia/ischemia and the inhibition of mRNA expression of Hif1a and HIF1A-regulated Bnip3 after postreatment with DIM. Here, HIF1A is linked to asphyxia.