The factors with significant differences in univariate analysis were included in multivariate analysis, and the result suggested that the signature (HR 1.140, 95%CI 1.036‐1.254, P = .007), AFP (HR 1.675, 95%CI 1.026‐2.736, P = .039), and hepatitis B (HR 0.548, 95%CI 0.307‐0.976, P = .041) could predict HCC patients’ OS independently (Figure 3P). This evidence concerns the gene AFP and hepatitis B virus infection.