Similarly, the top ranked hub genes in other gene modules negatively correlated with CRC were also verified to be expressed significantly lower in colon cancer when compared with normal, such as DHDDS (P<1E-12), HIGD1A (P=1.67E-15), GIPC1 (P=4.98E-11), CNNM4 (P=8.66E-13) in the blue gene module, DNASE1L3 (P=1.63E-12) and CPA3 (P<1E-12) in the black gene module (data not shown). The gene discussed is CNNM4; the disease is malignant colon neoplasm.