Similarly, the top ranked hub genes in other gene modules negatively correlated with CRC were also verified to be expressed significantly lower in colon cancer when compared with normal, such as DHDDS (P<1E-12), HIGD1A (P=1.67E-15), GIPC1 (P=4.98E-11), CNNM4 (P=8.66E-13) in the blue gene module, DNASE1L3 (P=1.63E-12) and CPA3 (P<1E-12) in the black gene module (data not shown). Here, CPA3 is linked to malignant colon neoplasm.