In our ALI mice model, the expression of miR-377-3p enhanced in hucMSCs-exosomes and LPS co-treated lung tissues compared with LPS alone treated lung tissues, while the expression of RPTOR in hucMSCs-exosomes administrated lung tissues was decreased compared with LPS treated mice, indicating that activation of autophagy by hucMSCs-exosomes protected LPS induced ALI in vivo. The gene discussed is RPTOR; the disease is acute respiratory distress syndrome.