We next verified the role of HMBOX1 in vivo by injecting shNC, shWTAP, shHMBOX1, and shWTAP/shHMBOX1 U2OS cells to induce subcutaneous osteosarcoma mice model, orthotopic xenograft tumor model, and tail vein metastasis model. Here, HMBOX1 is linked to osteosarcoma.