Overall, RCC CD8+ TILs showed increased expression of many costimulatory and coinhibitory genes (PD-1, CTLA4, LAG3, TIM-3) and markers of activation 4-1BB [TNFRSF9], OX40L [TNFSF4], and CD70 [ligand of CD27]), as well as downregulation of CD3, CD8, and OX40 receptor (TNFRSF4) (Supplemental Figure 1C). This evidence concerns the gene LAG3 and renal cell carcinoma.