As shown in Figure 4E, all the hub genes were significant in distinguishing CPT11 and other treatments for colon cancers (P<0.05), among which Lrs1, Pls1, Ank2, Adam10, Grpel2, Emp2, Adil, Pafah2, Hey1 and Aif1l were up-regulated while Bloclsl, Psmb8, Ostc, Rtp4, Gm12250, Mrpl3, Gm12250, Mrpl3, Gmppb, Lfitm3, Gbp2 and Epstl1 were down-regulated. This evidence concerns the gene PLS1 and colonic neoplasm.