The results showed us that the red module mostly correlated with PHY906 treatment in colon cancer, and nine TFs (E2F1, PML, SIN3A, NANOG, USF1, FOXA1, GATA2, TCF3, and NFE2L2) were significantly associated with the genes within red module. The gene discussed is USF1; the disease is malignant colon neoplasm.