To examine whether these findings are applicable to AML cells, we KD CALM in an inducible manner (CLAM iKD) in MV4-11 (with FLT3 ITD), HMC-1 (with KIT D816V) and HL-60 (with WT-FLT3 and KIT) cells with a doxycycline (DOX) inducible system, in which CALM shRNA expression was induced by the addition of DOX into the culture medium. This evidence concerns the gene PICALM and acute myeloid leukemia.