Notably, Rubiconad−/− mice weighed less than controls fed an HFD (Supplementary Fig. 4a), and adipose-specific Rubicon-knockout mice fed an HFD exhibited reduced adipose weight (Supplementary Fig. 4b) and adipose cell size (Supplementary Fig. 4c–e), but an increase in glucose intolerance (Supplementary Fig. 4f) and hepatic steatosis (Supplementary Fig. 4g, h). Here, RUBCN is linked to Glucose intolerance.