ARHGEF16 and neoplasm: Then, the expression of ARHGEF16 in the xenograft tumors was determined by western blotting (Fig. 3d and Supplementary Fig. S1j).The overexpression of ARHGEF16 led to dramatic increases in the average tumor volume (by ∼6-fold; Fig. 3a, b) and the average tumor weight (by ∼3-fold; Fig. 3c and Supplementary Fig. S1h, i) compared with control expression.