We first analyzed cell proliferation by assessing colony formation and found that it was increased in ARHGEF16 + Sh-control cells relative to Vector + Sh-control cells, but this increase was abrogated by knocking down FYN expression (ARHGEF16 + Sh-FYN; Fig. 5a, b), suggesting that ARHGEF16-induced colon cancer cell proliferation was regulated by FYN. This evidence concerns the gene ARHGEF16 and colonic neoplasm.