The AAV-SB-CRISPR library was transduced into Cas9-expressing mouse CD8+ T cells, which were transplanted into mouse brain tumors; validation of the screen hits was performed by adoptive transfer of CD8+ T cells with Pdia3, Mgat5, Emp1, or Lag3 knockouts, which improved survival of GBM-bearing mice [162]. Here, CD8A is linked to glioblastoma.