SOD1 and amyotrophic lateral sclerosis: Successful detection of misfolded SOD1 in ALS tissues with a lower dilution rate of the antibodies was reported by Grad et al. and Pokrishevsky et al., but Da Cruz et al. appear to have used a significantly diluted solution of the antibody and failed to detect the non-SOD1 ALS-specific, 3H1- and 10E11C11-positive staining.