Amongst these samples, TP53 was the most commonly altered at 40%, followed by PIK3CA, CCND1, NCOR1 and FGFR1. In order to identify any gene that may be enriched in the metastatic cohort, the mutational frequencies were compared to those in the TCGA breast cancer dataset comprised of primary tumours [31]. The gene discussed is TP53; the disease is breast cancer.