Because NAFLD in children has no specific clinical manifestations, and ultrasound is not sensitive to the diagnosis of NASH, further studies can be conducted to evaluate whether PNPLA3 738,409 G/C gene polymorphism can be screened for early diagnosis of childhood NAFLD and early evaluation of NAFLD severity. The gene discussed is PNPLA3; the disease is metabolic dysfunction-associated steatotic liver disease.