Based on the designation of “N intensity +”, which is associated with higher risk of cancer, CRCs were associated with positive status for nuclear CTNNB1 intensity (age, gender adjusted OR: 3.40, 95%CI = 1.42–8.15, P = 0.006 for positive nuclear staining) compared to non-CRC samples (Normal or advanced adenoma) (Fig. 1). Here, CTNNB1 is linked to cancer.