AIFM2 and infection: However, its single progeny, isolate 3363, carried a missense variant in amidase (amiD) gene essential for protein translation and required for mycobacterial survival during infection (virulence factor) and a frameshift deletion in the thioesterase region of polyketide synthase 13 (pks13) gene involved in the final assembly of mycolic acids, major and specific lipid components of the envelope essential for the survival of a mycobacterial cell [40, 42].