Changes in gene expression which did correlate with functional TNKSi sensitivity included reductions in the expression of the intestinal stem cell marker LGR5 and the upregulation of cytokeratin 20 (KRT20) (Fig 4B; S6 Fig), suggesting that tankyrase inhibition may induce differentiation as has previously been shown with CRC cell lines [8]. The gene discussed is TNKS; the disease is colorectal carcinoma.