We reported the tightly coordinated expression of dmyc, miR-277, imd,Tab2 and Dpt, suggesting that dMyc as a novel negative regulator primarily prevents the over-activation of Drosophila Imd immune response at this middle and later stage of E. coli infection, and helps Drosophila restore to a new immune homeostasis. Here, DPT is linked to escherichia coli infection.