In semi-quantitative assay using immunostaining pictures, the average of percentage areas positive for involucrin immunostaining in adequate area (800–900μm2) of epidermis were 4.9% for HR-AD group, 8.5% for Control group, 16.9% for CAEP group, and 11.6% for GluCer group (n = 2). This evidence concerns the gene IVL and Alzheimer disease.