VHL and neoplasm: Since the intronic sequencing reads do not start at the same position in different tumour samples, it is difficult to assess the effect of this read-through on potential translation of the resulting mRNA transcript, however western blotting of primary cells derived from Vhlfl/fl mice demonstrated that Cre-mediated recombination results in complete loss of the pVHL protein isoforms15 and Supplementary Fig. 5b.