TSC2 and Uterine leiomyoma: To determine whether rapamycin affects cell proliferation and death in vitro, we use TSC2-deficient patient-derived cells [27, 28], Tsc2-deficient rat uterine leiomyoma-derived cells [24, 25], and Tsc2−/−p53−/− mouse embryonic fibroblasts (MEF) and their TSC2-exressing counterpart controls [29].