Pretreatment of 5 μM concentration of genistin was used against astrocytes and it protects by working on interleukin 1 beta (IL-1β), tumor necrosis factor-α (TNF-α), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) cascade, and reversing the effect of NDs in the AD model [38]. Here, TNF is linked to Alzheimer disease.