Analysis using publicly available algorithms (Target Scan, miRWalk, and miRanda) showed that a number of negative regulators, including RICTOR, SKI, NDP, SMAD7, IGF1R, CCND1, RAD9A, TIMP3, FN1, XOH, PTPN9, ABCB1, ADCY1, CUL3, SUV39H1, ITGA5, and EZR were associated with ESCC. This evidence concerns the gene EZR and esophageal squamous cell carcinoma.