Analysis using publicly available algorithms (Target Scan, miRWalk, and miRanda) showed that a number of negative regulators, including RICTOR, SKI, NDP, SMAD7, IGF1R, CCND1, RAD9A, TIMP3, FN1, XOH, PTPN9, ABCB1, ADCY1, CUL3, SUV39H1, ITGA5, and EZR were associated with ESCC. Here, TIMP3 is linked to esophageal squamous cell carcinoma.