Interestingly, these groups developed fatty liver associated with an increase in the expression of lipogenic genes, particularly sterol regulatory element binding protein (SREBP-1) and fatty acid synthase (FAS), and an increase in the expression of the gluconeogenic gene phosphoenolpyruvate carboxykinase (PEPCK), particularly in the groups fed sucrose or sucralose (Figure S5A-E), whereas the brown sugar+HFD and honey+HFD groups showed SCFA concentrations similar to those in the C group. The gene discussed is FAS; the disease is fatty liver disease.