To gain more insights into CD5high and CD5low subpopulations in IgHVmut and IgHVunmut patients, we constructed the multivariate PSNs and performed their clustering based on the CXCR3, CXCR4, CXCR5, and CCR7 expression in enrolled CLL patients. The gene discussed is CXCR5; the disease is B-cell chronic lymphocytic leukemia.