To further investigate the TIGIT/DNAM-1 axis in AML patients, we further examined DNAM-1 expression on γδ Treg subsets and found that the expression of DNAM-1 was largely concentrated in γδ Treg populations in AML-NR and de novo patients, which may be related to T cell activation status. This evidence concerns the gene CD226 and acute myeloid leukemia.